Our History

Rhamnolipid Companies, Inc. in conjunction with Rhamnolipid, Inc. has been researching since 2003 for a cost-efficient method for the production of rhamnolipid.

In our quest for cutting edge rhamnolipid production, we were meet with many obstacles, but we kept at it. We encountered non-producer strains, like others, we encountered the problem of by-products and the dreaded foaming problem.

In 2008, we started to gain success and developed a non-toxic trade secret that eliminates the over-foaming issue with our rhamnolipid production.

In 2012, we meet the substrate dilemma. The substrate is what we feed the rhamnolipid to grow. Buying olive oil in bulk (with no preservatives) led to a very expensive production process. In 2014, we replaced the substrate with a food source that is clean (less clean-up which reduces time) and cost efficient to buy in industrial quantities. In 2016, we developed a production process that reduces the tank time from 14 days to 5 days.

In 2018, we signed contracts with companies to provide samples through our rhamnolipid stores. One group provides rhamnolipid through store.rhamnolipid.com utilizing several different languages. Another group manages the North, Central and South America inquiries through www.rhamnolipids.net.


Rhamnolipid applications are registered with the United States Environmental Protection Agency (RhamnoWash), Food and Drug Administration (FDA) and the Florida Environmental Protection Agency. In the latter half of 2017, new discoveries have been made regarding agriculture, pharmaceutical, and environmental. Rhamnolipid is made up of di-rhamnolipid and mono-rhamnolipid, therefore, the correct use of rhamnolipid requires the understanding of ratios of mono-rhamnolipid to di-rhamnolipid. As important as ratios are, understanding purity, dilution rates and carriers leads to successful results in the field. Through all our testing, we made our share of mistakes. The application of rhamnolipid has validated many third-party publications which showcases how unique the rhamnolipid molecule is. With the correct knowledge, our rhamnolipid has shown to be a replacement for synthetic chemicals that cause a toxic environment. Some successful applications we have worked with include:

Removal of hydro-carbons with Rhamnowash, soil washing to remove oil, metal sequestering in soil washing- rhamnolipid produces a stronger bind with metals than soil does,

Pest control on crops -rhamnolipid destroyed plant pathogens,

Mosquito repellent, larvicide and pesticide -Pharmaceutical anti-bacterial application- Liposomes, and many more


Our products are GMP and made with many different substrates. Rhamnolipid has many applications and our substrate technology enables us to replace surfactants being currently used with our biosurfactant.

We are proud to provide sample support with your current research in different languages.

Once the fermentation tanks are online, we can provide our clients with large scale production at cost-efficient prices.

One of our closely-held trade secrets is a producer strain that at current levels can produce highly purified rhamnolipid to the pharmaceutical industry for particular applications.

Another product we are proud to disclose is the application of rhamnolipid and citrus defense. Citrus Defence©, with rhamnolipid sprayed on fruits and vegetables will inhibit spoilage through the killing of damaging spores. The rhamnolipid will also help in the spreading of vegetable waxes used to reduce the loss of water during fruit and vegetable storage. Since rhamnolipid is approved as a food ingredient, rhamnolipids can be safely used on plants and fresh fruits and vegetables. Since rhamnolipid is natural and organic, their use will still allow the produce to be marked as organically grown and processed.